Induction of apoptosis and oxidative stress in estrogen receptor-negative breast cancer, MDA-MB231 cells, by ethanolic mango seed extract.
Từ khóa
trừu tượng
BACKGROUND
In this study, the effect of mango kernel extract in the induction of apoptosis of the breast cancer (MDA-MB-231) cell line was examined. This is an attempt to discover alternatives to current therapeutic regimes in the treatment of breast cancers.
METHODS
The pro-apoptotic markers, Bax, cytochrome c, caspases-. -8 and -9, and anti-apoptotic markers, Bcl-2, p53 and glutathione were determined in MDA-MB231 cells treated for 12 and 24 h with mango kernel extract.
RESULTS
The results showed that the extract produced a time- and dose-dependent increases in pro-apoptotic proteins and oxidative stress markers with a corresponding decrease in anti-apoptotic markers.
CONCLUSIONS
Based on the findings, mango kernel extract modulates redox balance in MDA-MB-231 breast cancer cells with a tendency for apoptotic cell death. The changes observed in this study may collectively underlie the basis for the cell death induced in MDA-MB-231 breast cancer cells by mango kernel extract. Thus, mango kernel extract has potential to be developed into an antibreast cancer mixture, and hence these results are worth studying further.
