Isoproturon-induced salicylic acid confers Arabidopsis resistance to isoproturon phytotoxicity and degradation in plants.
Từ khóa
trừu tượng
This study identified the effect of salicylic acid on degradation of isoproturon in Arabidopsis. Three T-DNA insertion mutant lines pal1-1, pal1-2 and eps1-1 defective in salicylic acid synthesis were tested, which showed higher isoproturon accumulation and toxic symptom in the mutants. When treated with 5 mg/L salicylic acid, these lines displayed a lower level of isoproturon and showed attenuated toxic symptom. RNA-sequencing study identified 2651 (1421 up and 1230 down) differentially expressed genes (DEGs) in eps1-1 and 2211 (1556 up and 655 down) in pal1-2 mutant plants (> 2.0 fold change, p< 0.05). Some of the DEGs covered Phase IIII reaction components like glycosyltransferases (GTs) and ATP-binding cassette transporters (ABCs). Using ultra performance liquid chromatography-time of fight tandem-mass spectrometer/mass spectrometer (UPLC-TOF-MS/MS), thirteen Phase I and four Phase II metabolites were characterized. Of these, two metabolites 1-OH-isopropyl-benzene-O-glucoside and 4-isopropylphenol-S-2-methylbutanoyl-serine have been identified and reported for the first time.