[Studies on the chemical components of Nelumbinis Plumula and the inhibitory activity on protein disulfide isomerase].

Increasing evidence suggested that protein disulfide isomerase supported the survival and progression of several cancers. Nelumbinis Plumula is a Chinese traditional herb which showed antitumor activity. To find if the Nelumbinis Plumula affect protein disulfide isomerase activity, we studied its chemical constituents, and 12 monomeric compounds were isolated by means of solvent extraction, silica gel column chromatography, preparative HPLC and recrystallization. Among them, N-methylcoclaurine, kaempferol, chrysoeriol-7-O-neohesperidoside and mannitol were obtained for the first time. Following, we tested the compounds inhibitory activity on protein disulfide isomerase. The results showed that N-methylcoclaurine, neferine, liensinine and isoliensinine could inhibit the activity of protein disulfide isomerase in vitro, their IC₅₀ values were 1.4, 2.9, 4.0 and 5.4 μmol•L⁻¹, respectively.