Biomineralized Biohybrid-Algae for Tumor Hypoxia Modulation and Cascade Radio-Photodynamic Therapy
Từ khóa
trừu tượng
The biomineralization of biomaterials has shown extraordinary potential in cancer treatment, but the exploration of their in vivo applications is still insufficient. Here, we report a biohybrid microalgae system using a biomineralization approach to improve their biocompatibility, while keeping their living activities for radiation and photodynamic synergistic therapy in breast cancer. The biohybrid-algae (Algae@SiO2) synthesized by a one-step biomimetic silicification method could significantly enhanced their cytotoxicity and tolerance, improve the living activity in the tumor area. The innate of chlorophyll and unique optical property make the Algae@SiO2 possess dual imaging ability, including photoacoustic imaging (PAI) and fluorescence imaging (FI). Algae@SiO2 accumulated in tumor sites could generate oxygen in situ by the external light-mediated photosynthesis, relieve the tumor hypoxia, then enhance the efficiency of radiation therapy. As a natural photosensitizer, the released chlorophyll from Algae@SiO2 could provide reactive oxygen species (ROS) to generate cancer cell apoptosis for the cascaded photodynamic therapy. The significant suppression of tumor growth and metastasis in the 4T1 breast tumor-bearing mice successfully demonstrate the promising anti-tumor effect of the Algae@SiO2 mediated synergistic therapy. Our results show that biohybrid-algae, integrated with PAI/FI dual imaging, radiosensitization and cascaded photothermal therapy, is a promising multifunctional efficient biosystem for cancer treatment.