Enzymatic degradation of Ginkgolic acid by Laccase Immobilized on Novel Electrospunned Nanofiber Mat.

Ginkgo biloba leaf extract contains many health beneficial active ingredients. However, ginkgolic acid, one of the major components found in G. biloba extract, may cause serious allergic and toxic side effects to the living organisms. The purpose of this study is to immobilize the laccase system on the electrospun nylon fiber mat (NFM) to efficiently hydrolyze the ginkgolic acid within G. biloba leaf extract.Novel electrospinning technology successfully produced high-quality nanoscopic fiber mats made of the mixture of multi-walled carbon nanotube and nylon 6,6. Laccase that was immobilized onto the NFM exhibited much higher efficiency in the catalyzation of ABTS than nylon 6,6 pellet. After being immobilized onto the NFM, the pH and temperature stability of laccase were significantly improved. The nanofiber mat immobilized laccase could maintain more than 50% of its original activity even after 40 days of storage or 10 operational cycles. The kinetic parameters, including rate constant (K), the time (τ50) in which 50% of ginkgolic acid hydrolysis was reached, the time (τcomplete) required to achieve complete ginkgolic acid hydrolysis, Km and Vmax were determined, which were 0.07 ± 0.01 min - 1, 8.97 ± 0.55 min, 45.45 ± 2.79 min, 0.51 ± 0.09 mM and 0.49 ± 0.03 mM/min*mg, respectively.The result successfully demonstrated the great potential of using novel electrospun nanofiber mat as enzyme immobilization platform, which could significantly enhance of the enzyme activity and stability. This article is protected by copyright. All rights reserved.