In vivo the antioxidative extract of Averrhoa carambola Linn. leaves induced apoptosis in Ehrilch ascites carcinoma by modulating p53 expression

This study was designed to evaluate the antioxidant activity of methanol extract of Averrhoa carambolla Linn. leaves (MELA) using DPPH^· and ABTS^·+ free radical scavenging assays whereas its antineoplastic effect against Ehrlich ascites carcinoma (EAC) was assed using viable cell count, life span, body weight gain and hematological parameters of experimental mice. Results showed that rich phenolic and flavonoid content of MELA had moderate dose dependent free radical scavenging activity (IC₅₀: 62.0 μg/mL for DPPH^· and 6.0 μg/mL for ABTS^·+). In vivo antineoplastic assay, MELA significantly (P < 0.05) decreased viable cells and body weight gain, increased the survival time and restored altered hematological profiles of cancer cell bearing mice. Fluorescence microscopic view of EAC cells derived from MELA-treated group showed apoptotic characteristics and this observation was also supported by overexpression of pro-apoptotic genes coding p53 and Bax proteins in treated cancer cells. The anti-apoptotic genes coding Bcl-2 protein was also absent in treated EAC cells as compared with the control. Moreover, phytochemical profiles of MELA as identified by GC/MS analysis are also consistent with its activities.

Keywords: Antineoplastic; Antioxidant; Apoptosis; Averrhoa carambolla; Leaves.