A three-step screening of selection for resistance against pathogenic bacteria infection, selection for resistance against pathogenic fungi infection, and selection for sensitivity to salicylic acid was carried out on Arabidopsis thaliana lines highly expressing rice full-length cDNA (rice-FOX Arabidopsis lines) which were prepared by using a FOX hunting system. As a result, one Arabidopsis thaliana line (line K15424) selected in all of the three types of screening was successfully selected. Line K15424 carries a rice full-length cDNA. Rice overexpressing AK070024 was produced, and resistance against rice bacterial leaf blight was assayed using the T1 generation. As a result, it was confirmed that AK070024-overexpressing rice is resistant against bacterial leaf blight and blast.

Mori; Masaki

Hayashi; Nagao

Sugano; Shoji

Takatsuji; Hiroshi

Hirochika; Hirohiko

Oda; Kenji

Matsui; Minami

National Institute of Agrobiological Sciences

Riken

Rice gene capable of imparting wide-spectrum disease resistance

PAD4 compositions from Arabidopsis, including genomic and cDNA nucleic acid sequences, and methods for using said compositions in plants are provided. PAD4 from Arabidopsis is demonstrated to be regulatory and is required upstream from salicylic acid in the signal transduction pathway leading from infection to activation of defense responses.

salicylic acid/arabidopsis

Rice gene capable of imparting wide-spectrum disease resistance

PAD4 nucleic acid compositions from Arabidopsis and methods therefor

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