Anti-inflammatory constituents from Perovskia atriplicifolia.
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BACKGROUND
Perovskia atriplicifolia Benth (Labiantae) has long been used as a traditional herbal medicine for anti-inflammation in Pakistan; this prompted us to isolate anti-inflammatory compounds from this plant.
OBJECTIVE
The objective of this study was to isolate and characterize the anti-inflammatory principles from Perovskia atriplicifolia.
METHODS
The CHCl3-soluble fraction of the methanol extract of the whole plant on column chromatography yielded compounds 1-6. The anti-inflammatory potential of the compounds 1-6 was evaluated by Leukotriene C4 (LTC4) Release Assay which was performed according to the established protocol. LTC4 in the supernatant of each well was measured using an ELISA kit (Cayman Chemical Co., Ann Arbor, MI).
RESULTS
The bioassay-guided phytochemical investigation of the CHCl3 soluble fraction of the methanol extract of Perovskia atriplicifolia furnished six compounds, abrotanone (1), abrotandiol (2), (+)-pinoresinol (3), (+)-syringaresinol (4), (+)-lariciresinol (5), and (+)-taxiresinol (6). The compounds (1-6) were evaluated for their inhibitory activities on LTC4 release. Among the tested compounds, (+)-taxiresinol (6) exhibited the most potent inhibition of LTC4 release with an IC50 value of 3.4 ± 0.09 µM followed by compounds 4, 5, 3, and 2 with an IC50 value ranging from 7.9 ± 0.04 to 17.2 ± 0.07 µM. Abrotanone (1) showed the lowest inhibition of LTC4 release with an IC50 value of 35.1 ± 0.05 µM (the positive control, zileuton, 0.77 ± 0.05 µM).
CONCLUSIONS
Compounds 1-6 were found to possess inhibitory activity and seem to have potential therapeutic effect on inflammatory diseases.
