Boron Deficiency Increases Cytosolic Ca2+ Levels Mainly via Ca2+ Influx from the Apoplast in Arabidopsis thaliana Roots.

Boron (B) is a micronutrient for plant development, and its deficiency alters many physiological processes. However, the current knowledge on how plants are able to sense the B-starvation signal is still very limited. Recently, it has been reported that B deprivation induces an increase in cytosolic calcium concentration ([Ca²⁺]_cyt) in Arabidopsis thaliana roots. The aim of this work was to research in Arabidopsis whether [Ca²⁺]_cyt is restored to initial levels when B is resupplied and elucidate whether apoplastic Ca²⁺ is the major source for B-deficiency-induced rise in [Ca²⁺]_cyt. The use of chemical compounds affecting Ca²⁺ homeostasis showed that the rise in root [Ca²⁺]_cyt induced by B deficiency was predominantly owed to Ca²⁺ influx from the apoplast through plasma membrane Ca²⁺ channels in an IP₃-independent manner. Furthermore, B resupply restored the root [Ca²⁺]_cyt. Interestingly, expression levels of genes encoding Ca²⁺ transporters (ACA10, plasma membrane P_IIB-type Ca²⁺-ATPase; and CAX3, vacuolar cation/proton exchanger) were upregulated by ethylene glycol tetraacetic acid (EGTA) and abscisic acid (ABA). The results pointed out that ACA10, and especially CAX3, would play a major role in the restoration of Ca²⁺ homeostasis after 24 h of B deficiency.