Applied Immunohistochemistry and Molecular Morphology 2008-Oct
Immunohistochemistry on frozen section of sentinel lymph nodes in breast cancer with improved morphology and blocking of endogenous peroxidase.
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Sentinel lymph node biopsy in the management of patients with breast cancer is the clinical practice. Peroperative examination means that more patients can be treated in a 1-step procedure. The addition of immunohistochemistry to frozen section slides improves the detection rate of especially micrometastasis. We present a novel method for immunohistochemical staining on a frozen section material that gives better morphology and blocks endogenous peroxidase sufficiently.