Toxicological effect of Parthenium hysterophorus and milk processing industry sludge on earthworms, Eisenia fetida.

Production of large quantities of organic waste all over the world poses major environmental and disposal problems. The present study was conducted to explore the deleterious effects of Parthenium hysterophorus and milk processing industry sludge on the health of earthworm, Eisenia fetida. Earthworms were allowed to grow in the mixture of cow dung:Parthenium hysterophorus (75:25) and cow dung:milk processing industry sludge (60:40) for 60 days. The biochemical markers viz. superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) levels and histological changes in earthworm's intestine were assessed after 15, 30, 45, and 60 days of exposure. The results revealed increased MDA level, while SOD, CAT, and GPx activities showed variation in both treatments. Furthermore, histopathological changes revealed damage in the intestinal tissue in both treatments during all intervals. More severe effects were registered in P. hysterophorus treatment. Obtained results may contribute to the understanding of P. hysterophorus and milk processing industry sludge induced toxic effects on earthworms and to identify defense mechanism of Eisenia fetida.