Evaluation of the antioxidant and antidiabetic potential of the Poly Herbal Formulation. Identification of bioactive factors.

The present investigation is intended to prepare a poly herbal formulation (PHF) with Piper nigrum (fruits), Terminalia paniculata (bark) and Bauhinia purpurea (bark) and assess their antioxidant and glucose-lowering effects utilizing in vitro models.The individual plant methanolic extracts and PHF are exposed to phytochemical examination and to distinguish the bioactive factors by GC-MS. We assessed the antioxidant properties of individual plant extracts and the PHF by using DPPH scavenging method, H2O2 scavenging assay, TBARS assay and total antioxidant estimation. Likewise, the anti-diabetic activity was assessed by ɑ-amylase and α-glucosidase enzyme inhibition and glucose diffusion inhibitory techniques.We found that PHF contains a high measure of total phenolics, total flavonoids and tannin compared to individual plant extracts. The bioactive components were identified by the GC-MS. We also found that PHF had significantly higher antioxidant and glucose-lowering effects than the individual plant concentrates.In conclusion, it could be reasoned that due to the nearness of antioxidant components, the PHF has good potential in the administration of hyperglycemia, diabetes and the related state of oxidative stress. This study shows that PHF is superior to individual plant extracts, supporting the conventional PHF concept.