[Unsaturated fatty acid of Actinidia chinesis planch seed oil (kiwi fruit essence) inhibits growth and metastasis of transplanted tumor in lung adenocarcinoma mice by up-regulating EHD2 expression]

Objective To observe the role of Eps15 homology domain containing protein 2 (EHD2) in the inhibitive effects of unsaturated fatty acid of Actinidia chinesis planch seed oil (kiwi fruit essence) on the growth and metastasis of transplanted tumor in lung adenocarcinoma mice. Methods 32 C57BL/6J mice bearing Lewis lung adenocarcinoma cells were randomly divided into the control group, 60, 120 and 240 mg/kg kiwi fruit essence treatment groups. Each group included 8 animals. From the fourth day after innoculation, the mice in the control group were intragastrically treated with normal saline, and the mice were intragastrically treated with 60, 120 or 240 mg/kg kiwi fruit essence in the corresponding kiwi fruit essence treatment groups. After measuring the volume of transplanted tumors, all mice were sacrificed on day 24, and their pulmonary tissues were then removed to observe tumor metastasis. The transplanted tumors were exfoliated and weighed to calculate the metastasis inhibition rate and tumor inhibition rate. The protein expression level of EHD2 in the transplanted tumors was detected by immunohistochemistry and Western blotting. The mRNA expression level of EHD2 in the transplanted tumors was measured using real-time quantitative PCR. Results Compared with the control group, the growth, volume, quality and number of lung metastasis nodules of the subcutaneous transplanted tumors significantly decreased, and tumor inhibition rates and metastasis inhibition rates increased in 60, 120, 240 mg/kg kiwi fruit essence treatment groups. The protein and mRNA level of EHD2 in the subcutaneous transplanted tumors went up. Compared with the 60 mg/kg kiwi fruit essence treatment group, the above indicators were significantly improved in 120 and 240 mg/kg kiwi fruit essence treatment groups in a dose-dependent manner. Conclusion Kiwi fruit essence can up-regulate EHD2 expression, thereby inhibiting the growth and metastasis of lung adenocarcinoma transplantation tumor in mice.