Melatonin improves K ⁺ and Na ⁺ homeostasis in rice under salt stress by mediated nitric oxide

Rice (Oryza sativa L.) productivity is greatly affected by soil salinity and melatonin (MLT) has long been recognized as a positive molecule that can alleviate the damage caused by salt. Here, the role of nitric oxide (NO) in the regulation of salt tolerance by MLT was investigated in rice. MLT pretreatment increased the fresh and dry weight of rice seedlings under salt stress. Its beneficial effects include less relative electrolyte leakage (REL) and better K⁺/Na⁺ homeostasis. MLT increased the activity of nitric oxide synthase (NOS). The polyamines (PAs) content and the utilization of arginine were also increased, thereby increasing NO content in salt-stressed rice seedlings. Pharmacological approach showed that NO, as a necessary downstream signaling molecule, was involved in the regulation of MLT on the K⁺/Na⁺ homeostasis of rice. Under salt stress, MLT improved the H⁺-pumps activities in plasma membrane (PM) and vacuole membrane (VM) in roots, MLT also increased the ATP content of rice roots by increasing the NO content of rice. Thus, the efflux of Na⁺ and the influx of K⁺ were promoted. When endogenous NO was scavenged, the regulation of K⁺/Na⁺ homeostasis by MLT was blocked. Therefore, MLT mediated K⁺/Na⁺ homeostasis of rice under salt stress by mediating NO.

Keywords: K(+)/Na(+) homeostasis; Melatonin; NO synthesis; Nitric oxide; Rice seedlings; Salt stress.