alizarin/edema

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Characteristics of the limb malformations induced by maternal exposure to cadmium in the mouse.

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Single doses of 2,3,4,6,8,10, and 15 mg/kg of cadmium chloride were administered (SC) to groups of MF1 mice on one of days 7 to 12 of gestation. Fetuses collected on day 18 were observed for limb malformations, and alizarin red-S stained skeletons were examined for their skeletal bases.

[The effect of basic fibroblast growth factor and epidermal growth factor on corneal endothelial cells preserved in mid-term preserving medium].

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OBJECTIVE To investigate the effect of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) on corneal endothelial cell preserved in mid-term preserving medium. METHODS Corneal rings were preserved in mid-term preserving medium in control group. bFGF (5 ng/ml), bFGF (20 ng/ml),

Recovery of Corneal Endothelial Cells from Periphery after Injury.

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BACKGROUND Wound healing of the endothelium occurs through cell enlargement and migration. However, the peripheral corneal endothelium may act as a cell resource for the recovery of corneal endothelium in endothelial injury. OBJECTIVE To investigate the recovery process of corneal endothelial cells

Experimental study of trabecular tissue repair for corneal defect in rabbits

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Aim: To investigate the mechanism and effect of trabecular tissue repair for corneal defect, and to provide a theoretical basis for its clinical application. Methods:

Sodium valproate augments spontaneous neural tube defects and axial skeletal malformations in TO mouse fetuses [corrected].

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The TO mouse exhibits a low incidence (3.65%) of spontaneous exencephaly at birth. The objectives of this study were to determine if sodium valproate (VPA) would augment this background frequency of exencephaly and to characterize its gross and histologic bases. Single doses of 200, 400, or 600

Supravital and vital staining of diseased corneal endothelium in whole-mount preparations.

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Whereas specular microscopy may provide valuable preoperative information about the status of the endothelial cells in clear corneas, in many diseased corneas that come to penetrating keratoplasty, stromal opacities or epithelial edema prevents determinations about the condition of the endothelium.

Effect of shaking of corneal endothelial preservation.

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OBJECTIVE To investigate the effect of shaking on corneal endothelial preservation. METHODS Thirty-six dog corneal buttons were obtained under standard eye bank procedure, after the animals had been sacrificed for cardiovascular experiments in the surgical department. They were equally divided into

Axial skeletal malformations associated with cranioschisis aperta and exencephaly. The result of experimental intervention after the neural tube closure in rats.

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Maternal administration of a single dose of cyclophosphamide (20 mg/kg) after the neural tube closure (on day 12) resulted in exencephaly and cranioschisis in 100% of rat fetuses at term. Extensive hemorrhages and edema were regularly associated with these defects. Alizarin-red stained skeletal

Transplantation of cultured bovine corneal endothelial cells to species with nonregenerative endothelium. The cat as an experimental model.

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The in vivo transplantation of cultured bovine corneal endothelial cells has been attempted in the cat. Cat corneas denuded of their endothelium were coated with bovine corneal endothelial cells previously maintained in tissue culture. When grafted back into cat recipients, the corneal buttons

Effects of phthalate acid esters on zebrafish larvae: Development and skeletal morphogenesis.

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This study evaluated the acute developmental toxicity of six priority phthalic acid esters (PAEs) including dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), di-2-ethylhexyl phthalate (DEHP), di-n-octyl phthalate (DNOP), and benzyl butyl phthalate (BBP) in zebrafish

Exencephaly and axial skeletal dysmorphogenesis induced by maternal exposure to cadmium in the mouse.

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To investigate the axial skeletal dysmorphogenesis associated with exencephaly and facial abnormalities, two doses of cadmium chloride (4 mg/kg and 6 mg/kg) were administered subcutaneously to MF1 mice on day 7 of gestation (sperm-positive day = day 0). Fetuses were collected on day 18. Gross

Evaluation and transplantation of corneas from pseudophakic donor eyes.

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OBJECTIVE To evaluate the quality of corneal donor tissue from pseudophakic eyes for transplantation. METHODS Only capsular-supported posterior chamber pseudophakia was studied. Forty-five pairs of donor eyes were assessed and evaluated by standard Minnesota Lions Eye Bank (MLEB) protocol.

Comparative developmental toxicity evaluation of di- n-hexyl phthalate and dicyclohexyl phthalate in rats.

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To investigate the effects of di- n-hexyl phthalate (DHP) and dicyclohexyl phthalate (DCHP) on the development of fetus and placenta in utero, pregnant rats were exposed to DHP or DCHP at dosages of 0, 20, 100, and 500 mg/kg bw/day, by gavage, on gestational days 6-19. Anogenital distance (AGD) and

Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy.

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To evaluate the therapeutic efficiency of tissue-engineered human corneal endothelia (TE-HCEs) on rabbit primary corneal endotheliopathy (PCEP), TE-HCEs reconstructed with monoclonal human corneal endothelial cells (mcHCECs) and modified denuded amniotic membranes (mdAMs) were transplanted into PCEP

Reduction of copper-induced histopathological alterations by calcium exposure in Nile tilapia (Oreochromis niloticus).

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This study was undertaken to determine whether calcium could render any protection against copper (Cu) toxicity in Nile tilapia with emphasis on histopathological and histochemical analysis. The copper LC(50) values for 24, 48, 72, and 96 h to tilapia in the laboratory were 210.27, 213.34, 193.30,

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