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Mutations in Arabidopsis thaliana genes involved in the tryptophan biosynthesis pathway affect root waving on tilted agar surfaces.

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Arabidopsis thaliana roots grow in a wavy pattern upon a slanted surface. A novel mutation in the anthranilate synthase alpha 1 (ASA1) gene, named trp5-2wvc1, and mutations in the tryptophan synthase alpha and beta 1 genes (trp3-1 and trp2-1, respectively) confer a compressed root wave phenotype on

An improved agar-plate method for studying root growth and response of Arabidopsis thaliana.

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Arabidopsis thaliana is a widely used model plant for plant biology research. Under traditional agar-plate culture system (TPG, traditional plant-growing), both plant shoots and roots are exposed to illumination, and roots are grown in sucrose-added medium. This is not a natural environment for the

Root architecture and morphometric analysis of Arabidopsis thaliana grown in Cd/Cu/Zn-gradient agar dishes: A new screening technique for studying plant response to metals.

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A new screening strategy using Petri dishes with a gradient of distances between germinating seeds and a metal-contaminated medium was used for studying alterations in root architecture and morphology of Arabidopsis thaliana treated with cadmium, copper and zinc at sub-toxic concentrations. Metal

Growth of Arabidopsis thaliana seedlings under water deficit studied by control of water potential in nutrient-agar media.

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We have characterized the growth responses of Arabidopsis thaliana seedlings to water deficit. To manipulate the water potential, we developed a method whereby the nutrient-agar medium could be supplemented with polyethylene glycol (PEG 8000); PEG was introduced into gelled media by diffusion, which

Early responses of mature Arabidopsis thaliana plants to reduced water potential in the agar-based polyethylene glycol infusion drought model.

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Drought is one of the most important environmental stressors resulting in increasing losses of crop plant productivity all over the world. Therefore, development of new approaches to increase the stress tolerance of crop plants is strongly desired. This requires precise and adequate modeling of

Selection of Agar Reagents for Medium Solidification Is a Critical Factor for Metal(loid) Sensitivity and Ionomic Profiles of Arabidopsis thaliana

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For researchers in the plant metal field, the agar reagent used for the solid plate medium is a problematic factor because application of different agar types and even a different lot of the same agar type can mask the plant metal-related phenotypes and impair the reproducibility. In this study, we

Dissecting the genetic control of natural variation in salt tolerance of Arabidopsis thaliana accessions.

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Many accessions (ecotypes) of Arabidopsis have been collected. Although few differences exist among their nucleotide sequences, these subtle differences induce large genetic variation in phenotypic traits such as stress tolerance and flowering time. To understand the natural variability in salt

Arabidopsis thaliana sku mutant seedlings show exaggerated surface-dependent alteration in root growth vector.

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Roots of wild-type Arabidopsis thaliana seedlings in the Wassilewskija (WS) and Landsberg erecta (Ler) ecotypes often grow aslant on vertical agar surfaces. Slanted root growth always occurs to the right of the gravity vector when the root is viewed through the agar surface, and is not observed in

A novel root gravitropism mutant of Arabidopsis thaliana exhibiting altered auxin physiology.

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A root gravitropism mutant was isolated from the DuPont Arabidopsis thaliana T-DNA insertional mutagenesis collection. This mutant has reduced root gravitropism, hence the name rgr1. Roots of rgr1 are shorter than those of wild-type, and they have reduced lateral root formation. In addition, roots

Streptomyces lividans inhibits the proliferation of the fungus Verticillium dahliae on seeds and roots of Arabidopsis thaliana.

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Verticillium wilt, a vascular disease in more than 200 dicotyledonous plants, is due to the ascomycete fungus Verticillium dahliae. As documented by video-microscopy, the soil bacterium Streptomyces lividans strongly reduces the germination of V. dahliae conidia, and the subsequent growth of hyphae.

Autophagy mediates hydrotropic response in Arabidopsis thaliana roots.

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This work shows that autophagy plays a key role in the hydrotropic curvature of Arabidopsis thaliana roots. An analysis of GFP-ATG8a transgenic plants showed that autophagosomes accumulated in the root curvature 2 h after the transfer of seedlings to Normal Medium-Water Stress Medium (NM-WSM).

Pollen and ovule development in Arabidopsis thaliana under spaceflight conditions.

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The development of pollen and ovules in Arabidopsis thaliana on the space shuttle 'Endeavour' (STS-54) was investigated. Plants were grown on nutrient agar for 14 days prior to loading into closed plant growth chambers that received light and temperature control inside the Plant Growth Unit flight

Hydrotropism in abscisic acid, wavy, and gravitropic mutants of Arabidopsis thaliana.

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We have developed experimental systems to study hydrotropism in seedling roots of Arabidopsis thaliana (L.) Heynh. Arabidopsis roots showed a strong curvature in response to a moisture gradient, established by applying 1% agar and a saturated solution of KCl or K(2)CO(3) in a closed chamber. In this

Large-scale protoplast isolation and regeneration of Arabidopsis thaliana.

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A simple method for large-scale isolation and regeneration of Arabidopsis thaliana green protoplasts utilizing a novel starting material is presented. This technique takes advantage of shooty/embryogenic masses regenerating from fresh or cultured roots. There is no time-consuming mechanical

Methods for grafting Arabidopsis thaliana and Eutrema salsugineum.

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Background

Grafting, an ancient agronomic technique, is an artificial mode of asexual reproduction in plants. Recently, grafting research has gradually shifted from modifying agronomic traits to the study of molecular mechanism. Grafting is an excellent tool to study long-range

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