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Identification of an UDP-glucose: Flavonol 3-O-glucosyl-transferase from cell suspension cultures of soybean (Glycine max L.).

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A glucosyltransferase, which catalyses the glucosylation of flavonols, using uridine diphosphate-D-glucose as glucose donor, has been isolated and purified about 5-10 fold from cell suspension cultures of soybean (Glycine max L., var. Mandarin). The pH optimum for this reaction was ca. 8.5 in

RNA-Seq Analysis of Differential Gene Expression Responding to Different Rhizobium Strains in Soybean (Glycine max) Roots.

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The root nodule symbiosis (RNS) between legume plants and rhizobia is the most efficient and productive source of nitrogen fixation, and has critical importance in agriculture and mesology. Soybean (Glycine max), one of the most important legume crops in the world, establishes a nitrogen-fixing

[Occurrence and localization of a flavonol-converting enzyme in plants].

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The occurrence and distribution of an enzyme converting flavonols to 2,3-dihydroxy flavanones has been measured in various plants and found to occur in all plants tested.In garbanzo bean, Cicer arietinum L., the enzyme is found mainly in roots, hypocotyls, epicotyls and cytoledons while the other

Accumulation of flavonols in response to ultraviolet-B irradiation in soybean is related to induction of flavanone 3-beta-hydroxylase and flavonol synthase.

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There are several branch points in the flavonoid synthesis pathway starting from chalcone. Among them, the hydroxylation of flavanone is a key step leading to flavonol and anthocyanin. The flavanone 3-beta-hydroxylase (GmF3H) gene was cloned from soybean (Glycine max cultivar Sinpaldal) and shown to

Functional characterization of a UDP-glucose:flavonoid 3-O-glucosyltransferase from the seed coat of black soybean (Glycine max (L.) Merr.).

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The seed coats of black soybean (Glycine max (L.) Merr.) accumulate red (cyanidin-), blue (delphinidin-), purple (petunidin-), and orange (pelargonidin-based) anthocyanins almost exclusively as 3-O-glucosides; however, the responsible enzyme has not been identified. In this study, the full-length

LDL-antioxidant pterocarpans from roots of Glycine max (L.) Merr.

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The methanolic root extract of Glycine max (L.) Merr. was chromatographed, which yielded 10 flavonoids, including three isoflavones 1-3, five pterocarpans 4-8, one flavonol 9, and one anthocyanidin 10. All isolated compounds were examined for LDL-antioxidant activities using four different assay

The Role of Flavonol Glycosides and Carotenoids in Protecting Soybean from Ultraviolet-B Damage.

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The increase in ultraviolet-B (UV-B; 0.290-0.320 [mu]m) radiation received by plants due to stratospheric ozone depletion heightens the importance of understanding UV-B tolerance. Photosynthetic tissue is believed to be protected from UV-B radiation by UV-B-absorbing compounds (e.g. flavonoids).

New flavonol triglycosides from the leaves of soybean cultivars.

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Soybean (Glycine max) is a major crop in the world. Three new flavonol 3-O-glycosides, kaempferol 3-O-alpha-L-rhamnopyranosyl-(1 --> 4)-[alpha-L-rhamnopyranosyl-(1 --> 6)-beta-D-galactopyranoside] (1), kaempferol 3-O-alpha-L-rhamnopyranosyl-(1 --> 4)-[beta-D-glucopyranosyl-(1 -->

Impact of elevated levels of atmospheric CO2 and herbivory on flavonoids of soybean (Glycine max Linnaeus).

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Atmospheric levels of carbon dioxide (CO2) have been increasing steadily over the last century. Plants grown under elevated CO2 conditions experience physiological changes, particularly in phytochemical content, that can influence their suitability as food for insects. Flavonoids are important plant

A single-base deletion in soybean flavonol synthase gene is associated with magenta flower color.

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The Wm locus of soybean [Glycine max (L.) Merr.] controls flower color. Dominant Wm and recessive wm allele of the locus produce purple and magenta flower, respectively. A putative full-length cDNA of flavonol synthase (FLS), gmfls1 was isolated by 5' RACE and end-to-end PCR from a cultivar Harosoy

Genome-Wide Identification and Functional Characterization of UDP-Glucosyltransferase Genes Involved in Flavonoid Biosynthesis in Glycine max.

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Flavonoids, natural products abundant in the model legume Glycine max, confer benefits to plants and to animal health. Flavonoids are present in soybean mainly as glycoconjugates. However, the mechanisms of biosynthesis of flavonoid glycosides are largely unknown in G. max. In the present study, 212

Kaempferol glycosides and enzymes of flavonol biosynthesis in leaves of a soybean strain with low photosynthetic rates.

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Soybean (Glycine max L.) strains which accumulate kaempferol 3-(2(G)-glucosylgentiobioside) in their leaves fix CO(2) at rates significantly lower than those lacking this compound (Buttery, Buzzell 1976 Crop Sci 16: 547-550), and kaempferol aglycone is a well known inhibitor of photosynthesis in

Leaf traits associated with flavonol glycoside genes in soybean.

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In the soybean (Glycine max (L.) Merr.), the gene combination Fg1 Fg3 is responsible for the glycosylation in the biosynthesis of kaempferol triglucoside (K9) in leaves. The presence of K9 is associated with reduction in chlorophyll content, specific leaf mass, photosynthetic rate and stomatal

Transcriptome changes in the phenylpropanoid pathway of Glycine max in response to Pseudomonas syringae infection.

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BACKGROUND Reports of plant molecular responses to pathogenic infections have pinpointed increases in activity of several genes of the phenylpropanoid pathway leading to the synthesis of lignin and flavonoids. The majority of those findings were derived from single gene studies and more recently

Physical characteristics and nutritional composition of some new soybean (Glycine max (L.) Merrill) genotypes.

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Soybean is a major source of high quality protein and oil and soybean seed quality is often determined by seed nutritional and antinutritional parameters. The objective of this study was to investigate the physical characteristics and nutritional composition of some new soybean genotypes. Hundred

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