Bioprospecting medicinal plants for antioxidant components.
Avainsanat
Abstrakti
OBJECTIVE
To evaluate antioxidant activities of seven medicinal plant species and their fractions, and to identify their phenolic compounds.
METHODS
Two extractions were processed and further fractionated by column chromatography to evaluate the concentration that inhibit 50% of 2,2'-azinobis (3-ethylbenzothiazoline-6-suslfonic acid, 1,1-diphenyl-2-picryl-hydrazyl radicals, and their ferric reducing antioxidant power. The identification of the fractions of phenolic compounds was done by ultra performance liquid chromatography.
RESULTS
The aqueous-acetone extracts of Feretia apodanthera and Ozoroa insignis exhibited the highest antioxidant potentials comparable to those of the standard quercetin. Their subsequently silica gel column fractionation showed three most active fractions from which the major constituents quercetin, myricetin, kampferol, rutin and isoquercetin were identified.
CONCLUSIONS
These plant species have potent antioxidant profiles and polyphenol compounds that may help to manage with radical related disease and aging.
