Role of whole plant extract of Nelumbo nucifera Gaertn in treatment of Thrombolysis.

This study aims to find out the components responsible for antithrombotic activity of Nelumbo nucifera.Petroleum Ether, Chloroform and Hydroalcoholic extracts of whole plant of Nelumbo nucifera (Lotus) were prepared and assessed for its Thrombolytic, Anti-Platelet Aggregation activity and bleeding time. The extracts were further analyzed through HPTLC and GC-MS. Statistical Study was prepared through ANOVA trailed by Tukey's several evaluation test.Hydroalcoholic extract showed the highest activity at the concentration of 400µg/ml in thrombolytic assay (42.03±5.76), anti-platelet aggregation Assay (57.93±1.68) and bleeding time (70.17±2.16) in comparison to Clopodigrel (33.76±3.43), Aspirin (66.55±1.86) and Aspirin (93.85±2.75) at the concentration of 100 µg/ml respectively. 25 peaks were identified through GC-MS, out of which, ferulic acid (14.2µ/g) and quercetin (5.4 µ/g) were active chemical compounds. HPTLC showed different chromatograms in hydro alcoholic extracts like (1) chlorogenic, (2) Quercetin, (3) Benzoic acid, (4) Caffeic acid, (5) Ferulic acid, (6) Kaempferol, (7) Gallic acid.Based on these findings, flavonoids present in hydroalcoholic extract may be developed into drug for clinical application for the treatment of thrombosis in patients.