Study Of Coleus Amboinicus Extract in Increasing of Transforming Growth Factor-β1 (TGF-β1) Concentration and Docking Prediction Quercetin Derivatives in 4X0M Receptor on Uric Acid-Induced in Rats

Background: Coleus amboinicus extracts are known to have anti-oxidant activity, anti-platelet aggregations, antibacterial, anticancer and anti-inflammation.

Objective: To evaluate Coleus amboinicus (CA) extracts in increasing of transforming growth factor-β1 concentration and molecular docking prediction of quercetin on receptor 4X0M (TGF-β1), measuring the levels of BUN, serum creatinine and Glutathione Peroxidase (GPx) on uric acid-induced rats.

Method: Fourty-two male Wistar rats (Rattus norvegicus), 3 months, 150-200 g were allocated into 3 groups (n=14). The control group received placebo (U-0), treatment group were administered orally with uric acid 1,5% and oxonic acid 2% (U1) and received 500 mg/kg bw of the CA extracts (U-2) respectively for 30 days. Blood serums collected for analysis of creatinine and BUN concentrations. All groups were sacrificed to collect kidney for measuring of GPx activity and TGF-β1 concentration was conducted by avidin-horseradish peroxidase (HRP) sandwich-Elisa. Kidney organ was collected to histopathological analyzed by HE and PAS staining.

Results: CA extract analyzed by TLC has a relative fraction of flavonoids, terpenes, saponins, polyphenols and alkaloids. Induction with uric acid has proven to causes acute renal failure with indicated of elevated BUN, serum creatinine concentration and necrotic lesions of tubular membrane in treatment groups. Treatment of CA extract at a dose of 500 mg/kg bw could increase of GPx and of TGF-β1 concentration significantly (p≤0.05). Quercetin as a marker compound of CA extract has stronger bind to the TGF-β1receptor (PDB code: 4X0M) than its of 3WA_601 ligand by in silico.

Conclusion: CA extract is proven to inhibit acute renal failure by increasing of TGF-β1concentration and has strong binding of its receptor.

Keywords: Coleus Amboinicus; Glutathione Peroxidase (GPx); Quercetin; Transforming Growth Factor-β1 (TGF-β1); UricAcid.