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[The influence of protoplast media on the tumor induction on kalanchoe leaves].
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Experiments concerning tumor transformation of plant cells or protoplasts need complete knowledge of the effects of media components. Here inhibiting effects of cellulase Onozuka, glucose, sucrose and mannitol (up to 0.3M), of tissue culture media, and of the antibiotic aureomycin on the tumor
Direct recovery of mangostins from Garcinia mangostana pericarps using cellulase-assisted aqueous micellar biphasic system with recyclable surfactant.
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The α- and γ-mangostins from Garcinia mangostana pericarps (GMP) exhibit antioxidant, anti-bacterial, anti-inflammatory and anti-tumor properties. The extraction yields α- and γ-mangostins are often limited by the presence of the GMP cell walls. Therefore, the extraction and recovery of mangostins
Effect of Enzymatic pre-treatment of microalgae extracts on their anti-tumor activity.
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There is an increasing need to find natural bioactive compounds for pharmaceutical applications, because they have less harmful side effects compared to their chemical alternatives. Microalgae (MA) have been identified as a promising source for these bioactive compounds, and this work aimed to
Stimulation effect of chitosan on the immunity of radiotherapy patients suffered from lung cancer.
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Water soluble chitosan (WSC), with low molecular weight, has many special biological, chemical, and physical properties, such as antifungal activity, antibacterial activity, and antitumor activity. In this study, the WSC was prepared by hydrolysis of the original chitosan with commercial cellulase,
Effects of extraction methods on the yield, chemical structure and anti-tumor activity of polysaccharides from Cordyceps gunnii mycelia.
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This study was to investigate the effects of different extraction methods on the yield, chemical structure and antitumor activity of polysaccharides from Cordyceps gunnii (C. gunnii) mycelia. Five extraction methods were used to extract crude polysaccharides (CPS), which include room-temperature
[Discovery of phytopathogenic bacteria 100 years ago: transatlantic controversies and polemics].
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The demonstration of a bacterial cause of some plant diseases has been claimed few years after it was commonly recognized that bacteria were able to cause diseases of human and animal. Nevertheless, some sharp controversies took place, between German and American specialists (1897-1901), before the
Cellulose Nanoparticles are a Biodegradable Photoacoustic Contrast Agent for Use in Living Mice.
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Molecular imaging with photoacoustic ultrasound is an emerging field that combines the spatial and temporal resolution of ultrasound with the contrast of optical imaging. However, there are few imaging agents that offer both high signal intensity and biodegradation into small molecules. Here we
Simultaneous extraction and biotransformation process to obtain high bioactivity phenolic compounds from Brazilian citrus residues.
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Recent studies have pointed to a reduction in the incidence of some cancers, diabetes, and neuro-degenerative diseases as a result of human health benefits from flavanones. Currently, flavanones are obtained by chemical synthesis or extraction from plants, and these processes are only produced in
In situ generation of tunable porosity gradients in hydrogel-based scaffolds for microfluidic cell culture.
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Compared with preformed anisotropic matrices, an anisotropic matrix that allows users to alter its properties and structure in situ after synthesis offers the important advantage of being able to mimic dynamic in vivo microenvironments, such as in tissues undergoing morphogenesis or in wounds
Exogenous carbohydrases added to a starter diet reduced markers of systemic immune activation and decreased Lactobacillus in weaned pigs1.
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Although the impact of carbohydrases on performance and nutrient utilization has been well studied, their effects on immune status and intestinal microbiota are less known in pigs. This study aimed to evaluate the impact of xylanase (X) and a carbohydrase enzyme blend (EB; cellulase, ß-glucanase,
Enhanced binding of modified pentosan polysulfate and heparin to bladder--a strategy for improved treatment of interstitial cystitis.
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OBJECTIVE
To attach galactosyl residues to pentosan polysulfate (PPS) and heparin so that these drugs will bind to the endogenous lectins in the bladder. The increased binding may improve their efficacy for treating interstitial cystitis.
METHODS
The anionic polysaccharides, PPS, and heparin were
Pretreatment of baicalin and wogonoside with glycoside hydrolase: a promising approach to enhance anticancer potential.
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Previous phytochemical studies showed that the major flavonoids in Scutellaria baicalensis are baicalin, baicalein, wogonoside and wogonin. The two glycosides (baicalin and wogonoside) can be transformed into their aglycons (baicalein and wogonin), which possess positive anticancer potential. In
Optimization of Ultrasonic-Assisted Enzymatic Extraction Conditions for Improving Total Phenolic Content, Antioxidant and Antitumor Activities In Vitro from Trapa quadrispinosa Roxb. Residues.
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Stems are the important residues of Trapa quadrispinosa Roxb., which are abundant in phenolic compounds. Ultrasonic-assisted enzymatic extraction (UAEE) is confirmed as a novel extraction technology with main advantages of enhancing extraction yield and physiological activities of the extracts from
Apoptosis-inducing activity of a driselase digest fraction of green tea residue.
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We enzymatically digested green tea residue with Driselase, a crude preparation containing cellulase, pectinase and proteases, in order to examine the potential usefulness of the residue. A fraction of the digest soluble in 70% ethanol was found to induce the death of U937 human histiocytic lymphoma
Purification and partial characterization of an acidic α-glucan-protein complex from the fruiting body of Pleurotus sajor-caju and its effect on macrophage activation.
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The aim of this study was to purify an acidic α-glucan-protein complex from the fruiting bodies of Pleurotus sajor-caju by using the cell wall-degrading enzymes, xylanase and cellulase. The acidic glucan-protein complex was separated from a polysaccharide extract by using DEAE Toyopearl 650M
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