Lotus (Nelumbo nucifera) seed protein isolate exerts anti-inflammatory and antioxidant effects in LPS-stimulated RAW264.7 macrophages via inhibiting NF-κB and MAPK pathways, and upregulating catalase activity.
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概要
Lotus seed has long been used in traditional medicine and cuisine. In this study, lotus seed protein (LSP) was isolated and evaluated its anti-inflammatory effect in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. LSP isolate (LSPI) treatment in LPS-stimulated RAW264.7 macrophages resulted in the significant (p < 0.05) decrease of NO production by downregulation of the expressions of mRNA and protein, including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, LSPI treatment attenuated the production of reactive oxygen species (ROS) through increasing catalase activity, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β in LPS-stimulated RAW264.7 macrophages. Furthermore, LPS stimulation in RAW264.7 macrophages caused the translocation of nuclear factor-kappa B (NF-κB) into the nucleus and the phosphorylation of mitogen-activated protein kinase (MAPK) but these stimulations were abolished by LSPI treatment.