The interplay of PsABAUGT1 with other abscisic acid metabolic genes in the regulation of ABA homeostasis during the development of pea seeds and germination in the presence of H2O2.

Inactivation of abscisic acid (ABA) in vitro may be catalyzed either by ABA 8'-hydroxylase (ABA8'OH) or by ABA uridine diphosphate glucosyltransferase (ABAUGT), which conjugates ABA with glucose. However, the involvement of these enzymes in the control of ABA content in vivo, especially ABAUGT, has not been fully elucidated. In pea seeds, both PsABAUGT1 and PsABA8'OH1 contribute to the reduction of ABA content during seed maturation and imbibition; however, during the first hours of imbibition, a high expression of only PsABAUGT1 was observed. Imbibition of seeds with H₂O₂ increased the ABA content despite the oxygen availability and altered the expression of metabolic genes. The expression of the biosynthetic gene 9-cis-epoxycarotene dioxygenase (PsNCED2) was increased, while that of PsABAUGT1 was decreased in each H₂O₂ experiment despite O₂ availability. Under hypoxia, only seeds imbibed with H₂O₂ germinated, while under nonlimiting oxygen conditions, the germination rate was not altered by H₂O₂. Under hypoxia, the germination rate of H₂O₂-imbibed seeds seemed to not depend on the absolute ABA content and rather on the balance between ABA and gibberellins (GA), as H₂O₂ increased the expression of GA synthesis genes. Overexpression of PsABAUGT1 in Arabidopsis decreases seed ABA content, accelerates germination and reduces seed sensitivity to exogenously applied ABA, confirming the ability of PsABAUGT1 to inactivate ABA. Thus, PsABAUGT1 is a new player in the regulation of ABA content in maturating and imbibed pea seeds, both under standard conditions and in response to H₂O₂.