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isothiocyanate/gorączka

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Fluorescein isothiocyanate staining intensity as a probe of hyperthermia-induced changes in chromatin conformation.

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In a previous report we presented evidence for large increases in fluorescein isothiocyanate (FITC) fluorescent intensity caused by hyperthermia which were not associated with synthesis of heat-shock proteins. We have now refined and considerably extended the measurements of increases in FITC

Effects of hyperthermia, irradiation, and cytotoxic drugs on fluorescein isothiocyanate staining intensity for flow cytofluorometry.

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Measurement of fluorescein isothiocyanate (FITC) staining intensity of cultured lymphoblastoid cells following hyperthermia showed large increases without concomitant increases in nuclear protein. Similar measurements of cells following incubation with cytotoxic drugs showed fluorescent intensity

Lipopolysaccharide challenge causes exaggerated fever and increased hepatic lipopolysaccharide uptake in vinblastine-induced leukopenic guinea pigs.

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OBJECTIVE To better understand the pathophysiology of the fever often manifested by immunocompromised patients undergoing chemotherapy that become neutropenic and suffer a bacterial infection. METHODS Prospective animal study. METHODS A physiology laboratory in a medical school setting. RESULTS We

Hyperthermia enhances the sensitivity of pancreatic cancer SW1990 cells to gemcitabine through ROS/JNK signaling.

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Pancreatic cancer (PC) is a highly aggressive type of cancer. Gemcitabine (GEM) is a standard chemotherapeutic treatment of advanced PC; however, it requires improvement, and more effective therapeutic methods must be further explored. In the present study, hyperthermia combined with GEM was used on

Spotted fever rickettsiae in ticks from the northern Sinai Governate, Egypt.

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A field study was initiated in 1988 to investigate whether spotted fever group rickettsiae occur in geographic areas in Egypt that are adjacent to an area in the southern Israeli Negev that has a defined focus of spotted fever disease. Ticks were collected from dogs, sheep, and camels at four study
Heat treatment of human erythrocytes led to increased passive cation permeability, followed by haemolysis. K+ leakage was linear up to a loss of about 80% in the temperature range 46-54 degrees C. Kinetic analysis of the results revealed an activation energy of 246 kJ/mol, implicating a transition

Regulation of immune activity by mild (fever-range) whole body hyperthermia: effects on epidermal Langerhans cells.

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Inflammation of the skin and systemic fever, both of which occur with injury or infection, include a hyperthermic component that many believe constitutes a physiological stress. Such increases in local or systemic body temperature may also have a regulatory effect on immune function. Langerhans

African swine fever. I. Antiserum production.

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These studies report on the production of African swine fever antiserum for use in serological tests. The first attempt to obtain antiserum was made by inoculating ASF virus - infected pig blood into the lactiferous sinus of lactating bovines. This failed to result in the development of detectable

Effect of hyperthermia on microvascular permeability to macromolecules in normal and tumor tissues.

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Microvascular permeability of macromolecules in normal and tumor tissues was measured under hyperthermic conditions. Fluorescein-isothiocyanate tagged dextran (150,000 molecular weight) was chosen as a tracer substance. The modified Sandison type rabbit ear chamber was used to study transport in

Immunofluorescent staining of the surfaces of lymphocytes in suspension from patients with dengue hemorrhagic fever.

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Immunofluorescent staining of lymphocytes suspensions from 55 of 62 patients with dengue hemorrhagic fever was positive for dengue antigen and human beta1C/a-globulin on the surface, from the second ay before shock or subsidence of fever. The percentages of positive staining of both components

The use of fluorescent-protein conjugates for staining brain capillaries in hypo- and hyperthermia (26-42 degrees).

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The temperature dependency of cerebrocortical capillary diameter (CD), number perfused with fluorescent tracer (CN), and intercapillary distance (ICD) has been investigated for three body temperatures (26, 37, and 42 degrees), in order to analyze the influence of undesired cooling or warming which

Magneto-plasmonic nanoparticles as theranostic platforms for magnetic resonance imaging, drug delivery and NIR hyperthermia applications.

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PEGylated magneto-plasmonic nanoparticles with a hollow or semi-hollow interior have been successfully synthesized and their physico-chemical characteristics have been investigated. The hollow interior space can be used to store drugs or other molecules of interest whereas magnetic characterization
The synthesis of Au@mesoporous SiO(2)/rhodamine B isothiocyanate (Au@mSiO(2)/RBITC) composite nanoparticles (NPs) is presented and their unique biofunctional properties are studied. The structure and morphology of the NPs are characterized by X-ray powder diffraction, transmission electron

Inhibitory effects of mild hyperthermia plus docetaxel therapy on ER(+/-) breast cancer cells and action mechanisms.

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The purpose of this study was to verify that a combination of mild hyperthermia and docetaxel chemotherapy produces synergistic antitumor effects and to explore the action mechanisms of this treatment approach. The effects of docetaxel on the proliferation of cells from the estrogen receptor
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