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Tumor-homing, size-tunable clustered nanoparticles for anticancer therapeutics.

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We present herein a pH-responsive dynamic DNA nanocluster based on gold nanoparticles with highly packed nucleic acid assembly and evaluate its potential as a drug delivery vehicle with tumor-specific accumulation. Each gold nanoparticle was readily functionalized with various functional DNA

[Observation on the therapeutic effect of neck clustered needling on insomnia].

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OBJECTIVE To compare therapeutic effects of neck clustered needling and medication on insomnia so as to search for a better method for treatment of insomnia. METHODS Seventy-six insomnia patients were randomly allocated to an acupuncture group and a medication group, 38 cases in each group. The

Stimuli-responsive clustered nanoparticles for improved tumor penetration and therapeutic efficacy.

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A principal goal of cancer nanomedicine is to deliver therapeutics effectively to cancer cells within solid tumors. However, there are a series of biological barriers that impede nanomedicine from reaching target cells. Here, we report a stimuli-responsive clustered nanoparticle to systematically

Visualization of clustered IgE epitopes on alpha-lactalbumin.

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BACKGROUND alpha-Lactalbumin (alpha-La) is a major cow's milk (CM) allergen responsible for allergic reactions in infants. OBJECTIVE We performed molecular, structural, and immunologic characterization of alpha-La. METHODS Recombinant alpha-lactalbumin (ralpha-La) was expressed in Escherichia coli,

Detection of clustered DNA lesions: Biological and clinical applications.

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Humans are daily exposed to background radiation and various sources of oxidative stress. My research has focused in the last 12 years on the effects of ionizing radiation on DNA, which is considered as the key target of radiation in the cell. Ionizing radiation and endogenous cellular oxidative

Hybrid Clustered Nanoparticles for Chemo-Antibacterial Combinatorial Cancer Therapy.

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Background: A great number of therapeutic limitations, such as chemoresistance, high dosage, and long treatments, are still present in cancer therapy, and are often followed by side effects such as infections, which represent the primary cause of death among patients. Methods: We

Intratumor performance and therapeutic efficacy of PAMAM dendrimers carried by clustered nanoparticles.

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In recent years, small nanoparticles (NPs) with diameter less than 10 nm have aroused considerable interests in biomedical applications. However, their intratumor performance, as well as the antitumor efficacy, has not been well understood due to their size-dependent pharmacokinetics, which presents

Early identification of unusually clustered mutations and root causes in therapeutic antibody development.

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This study reports findings of an unusual cluster of mutations spanning 22 bp (base pairs) in a monoclonal antibody expression vector. It was identified by two orthogonal methods: mass spectrometry on expressed protein and next-generation sequencing (NGS) on the plasmid DNA. While the initial NGS

Therapeutic Strategies of Clustered Regularly Interspaced Palindromic Repeats-Cas Systems for Different Viral Infections.

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The clustered regularly interspaced palindromic repeats (CRISPR)/Cas systems have rapidly transitioned from intriguing prokaryotic defense systems to an efficient therapeutic tool. This cutting-edge technology is currently revolutionizing to combat hostile viruses because of its reproducibility,

Self-Assembly of Therapeutic Peptide into Stimuli-Responsive Clustered Nanohybrids for Cancer-Targeted Therapy

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Clinical translation of therapeutic peptides, particularly those targeting intracellular protein-protein interactions (PPIs), has been hampered by their inefficacious cellular internalization in diseased tissue. Therapeutic peptides engineered into nanostructures with stable spatial architectures

Suppression of microRNAs by dual-targeting and clustered Tough Decoy inhibitors.

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MicroRNAs (miRNAs) are ubiquitous regulators of gene expression that contribute to almost any cellular process. Methods for managing of miRNA activity are attracting increasing attention in relation to diverse experimental and therapeutic applications. DNA-encoded miRNA inhibitors expressed from

Clustered burst firing in FMR1 premutation hippocampal neurons: amelioration with allopregnanolone.

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Premutation CGG repeat expansions (55-200 CGG repeats; preCGG) within the fragile X mental retardation 1 (FMR1) gene cause fragile X-associated tremor/ataxia syndrome (FXTAS). Defects in neuronal morphology and migration have been described in a preCGG mouse model. Mouse preCGG hippocampal neurons

Oral therapeutic agents with highly clustered globotriose for treatment of Shiga toxigenic Escherichia coli infections.

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Shiga toxin (Stx) is a major virulence factor in infection with Stx-producing Escherichia coli (STEC). We developed a series of linear polymers of acrylamide, each with a different density of trisaccharide of globotriaosylceramide (Gb3), which is a receptor for Stx, and identified Gb3 polymers with

Effects of dietary fibers on disturbances clustered in the metabolic syndrome.

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Because of its growing prevalence in Western countries, the metabolic syndrome, a common metabolic disorder that clusters a constellation of abnormalities, including central obesity, hypertension, dyslipidemia and insulin resistance, is emerging as one of the most important public health problems in

Voxel-based clustered imaging by multiparameter diffusion tensor images for glioma grading.

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Gliomas are the most common intra-axial primary brain tumour; therefore, predicting glioma grade would influence therapeutic strategies. Although several methods based on single or multiple parameters from diagnostic images exist, a definitive method for pre-operatively determining glioma grade

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