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Stran 1 iz 58 rezultatov
Identification of low potassium stress-responsive proteins in tobacco (Nicotiana tabacum) seedling roots using an iTRAQ-based analysis.
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Potassium is one of the three main mineral nutrients, and is vital for leaf growth and the quality of tobacco (Nicotiana tabacum) plants. In recent years, the isobaric tags for relative and absolute quantitation (iTRAQ) method has been one of the most popular techniques for quantitative proteomic
Determination of total ribonucleotide pool in plant materials by high-pH anion-exchange high-performance liquid chromatography following extraction with potassium hydroxide.
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A new, improved method that only requires a potassium hydroxide extraction procedure is presented for the analysis of a full nucleotide pool in plant materials. Quantification was performed by high-pH anion-exchange chromatography (HPAEC) with UV detection after a potassium hydroxide extraction, and
Functional characterization of a potassium transporter gene NrHAK1 in Nicotiana rustica.
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The purpose of this study is to investigate the function of a novel potassium transporter gene (NrHAK1) isolated from Nicotiana rustica roots using yeast complement and real-time PCR technique. The complementary DNA (cDNA) of NrHAK1, 2 488 bp long, contains an open reading frame (ORF) of 2 334 bp
[Difference of potassium absorption characters and mechanism of tobacco in genotypes].
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Tabacco (Nicotiana tabacum) has a relatively high requirement for potassium (K). The difference in root characteristics and ability to release K from minerals of flue-cured tobacco in four genotypes ND202, NC628, G28 and NC628 x ND202 were analyzed. The results showed that the more available K
Genome-wide analysis of the HAK potassium transporter gene family reveals asymmetrical evolution in tobacco (Nicotiana tabacum).
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Being an essential mineral nutrient, potassium (K+) plays numerous important roles in plant growth and development and determines the yield and quality of crop products. The cellular level of K+ is controlled to a large extent by the K+ transporter, which belongs to
Shaker-like potassium channels in Populus, regulated by the CBL-CIPK signal transduction pathway, increase tolerance to low-K+ stress.
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Shaker-like potassium channels in plants play an important role in potassium absorption and transport. Here, we characterized 11 genes encoding shaker-like channels from Populus trichocarpa. Furthermore, two homologs from this family were isolated from Populus euphratica and named PeKC1 and PeKC2.
The effects of sodium chloride, potassium chloride and glycerol on the activity of nitrate reductase of a salt-tolerant and two non-tolerant plants.
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The activity of nitrate reductase from the salt-tolerant alga Dunaliella parva is inhibited by sodium chloride and potassium chloride, but not by glycerol. The activity of the enzyme from Chlorella pyrenoidosa Chick 611-8b and from the XD line of tobacco (Nicotiana tabacum L. cv. Xanthi) cells is
Calcium channel antagonists induce direct inhibition of the outward rectifying potassium channel in tobacco protoplasts.
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Verapamil, nifedipine and bepridil, three antagonists of L-type calcium channels in animal cells, were shown to induce direct inhibition of outward rectifying potassium current in Nicotiana tabacum cv. Xanthi protoplasts with IC50 of 5 microM, 5 microM and 1 microM, respectively. In the outside-out
Vertical Profiles in Concentration of Potassium and Growth in Nicotiana tabacum. Effect of Potassium on Metabolic Proteins in Successive Leaves, and on the Direction of Translocation of (14)C-Photosynthate and its Allocation.
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In intact vegetative plants of N. tabacum grown with progressively rising supplies of potassium, the increase in concentration of the cation is greater in the basal region, and growth is promoted in leaves approaching their final weight and subtending basal nodes while it is restricted in the apical
Activation of potassium released from soil by root-secreted organic acids in different varieties of tobacco (Nicotiana tabacum).
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Organic acids secreted from the roots of plants play important roles in nutrient acquisition and metal detoxification; however, the precise underlying mechanisms of these processes remain poorly understood. In the present study we examined the content of organic acids exuded from roots and the
Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells.
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Potassium ion (K(+)) plays vital roles in many aspects of cellular homeostasis including competing with sodium ion (Na(+)) during potassium starvation and salt stress. Therefore, one way to engineer plant cells with improved salt tolerance is to enhance K(+) uptake activity of the cells, while
Transpiration, potassium uptake and flow in tobacco as affected by nitrogen forms and nutrient levels.
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OBJECTIVE
Ammonium can result in toxicity symptoms in many plants when it is supplied as the sole source of N. In this work, influences of different nitrogen forms at two levels (2 and 15 mm N) on growth, water relations and uptake and flow of potassium were studied in plants of Nicotiana tabacum 'K
Development of Nitrate Reductase Activity in Expanding Leaves of Nicotiana tabacum in Relation to the Concentration of Nitrate and Potassium.
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Up to 80% of the total nitrate reductase activity (NRA) determined in vivo in different parts of vegetative tobacco plant (Nicotiana tabacum) was located in the leaves. The NRA reached a peak when a leaf had expanded to 27% of its final weight and 33% of its final area. Thereafter, with advancing
Differential sodium and potassium transport selectivities of the rice OsHKT2;1 and OsHKT2;2 transporters in plant cells.
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Na(+) and K(+) homeostasis are crucial for plant growth and development. Two HKT transporter/channel classes have been characterized that mediate either Na(+) transport or Na(+) and K(+) transport when expressed in Xenopus laevis oocytes and yeast. However, the Na(+)/K(+) selectivities of the
Effect of potassium deficiency on tobacco mosaic virus multiplication in Turkish tobacco (Nicotiana tabacum).
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